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The dispensing material (chitosan and chitosan hydroxyapatite (HA) dissolved in acetic acid) was stored in a 30-ml barrel and forced out through a small Teflon-lined nozzle into a dispensing medium (sodium hydroxide ethanol in ratio of 7 3).
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GFP reporter assays were performed by diluting twice sub-cultured bacteria (OD595 ∼0.3) to OD595 ∼ 0.1 in DTA medium and dispensing 200 µl culture aliquots in a 96-well microplate.
NCI-H226 cells were prepared at a concentration of 100 000 cells/mL in RPMI growth medium before dispensing to a 96-well plate at densities varying from 500 cells/150 μL/well to 15 000 cells/150 μL/well.
By using multipipettor, 100 μl of broth medium dispensed into all wells of microtitre plate.
U-CH1 and U-CH2 cells were dispensed in culture medium at 500 to 1000 cells in 5 μL per well in 1536-well white/solid-bottom assay plates.
Cells were grown overnight on rich medium, dispensed into 96-well plates at a density of 10 cells per 200 μl, and grown.
The 92 J isogenic cell lines were dispensed in culture medium at 2,000 cells/5 μl/well in 1536-well white/solid-bottom assay plates.
An aliquot of 20 μL of 1 × 106 cfu/ml of the C. neoformans suspension was dispensed on the medium and then incubated in a dark humid chamber for 5 days, at 35°C.
Undifferentiated GCs were collected from the ovaries by puncturing follicles with a 25-gauge hypodermic needle, and cells were dispensed into 4F medium supplemented with 10 % FBS (GIBCO BRL, Grand Island, NY, USA), and incubated in a humidified atmosphere of 5%% CO2 at 37 °C.
The inoculum was prepared by dispensing 250 mL of Kühl medium in 500-mL Erlenmeyer flasks and then sterilized in an autoclave at 121°C and 1.5 atm.
For drug administration, Drosophila larvae were fed by dispensing the compound directly mixed with the nutritive medium.
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