Sentence examples for dispensing flow from inspiring English sources
The factors investigated included: culture ICD and duration, temperature, media pH, aspiration and dispensing flow rates, applied shear rate, trypsinization time, cell disaggregation conditions, relative centrifugal force, centrifugation time, and cell pellet resuspension rate.
Resuspension was by controlled aspiration cycles using an automated platform (Freedom Evo®75 Tecan, Männedorf, Switzerland) with pipette tips of 0.76 mm id, located 4.57 mm (i.e., 6× id) above the pellet surface and a dispensing flow rate of either 600 μL/s (1.3 m/s) or 900 μL/s (2.0 m/s).
The feed tank is connected to a high-pressure dosing pump that can dispense flow rates from 0.0 to 0.07 mm3 min−1.
As a result of these observations, various aspects of the trypsinization process were further investigated for optimization purposes including: trypsin incubation time, aspiration flow rate, dispense flow rate and number of pipette mixing steps employed.
Further investigation of the aspiration and dispense flow rates showed that an aspiration flow rate of 300 μL s−1 represented a threshold value, below which aggregate breakage was minimal, and above which no significant advantage was obtained (data not shown).
San Francisco Bay sediment slurries (SFB5; 20 mL) were dispensed under flowing N2 to serum bottles (160 mL total volume) containing 10 mL ABW and stoppered before flushing with oxygen-free N2 for an additional 5 min.
"Our remote NMR/MRI technology enables time-resolved imaging of multi-channel flow, dispenses with the need for large and expensive magnets for analysis, allows us to analyze complex and unprocessed mixtures in one pass, and adds portability to NMR/MRI," Bajaj says.
An aqueous solution of 10 mM H2SO4 was used as the eluting buffer and was dispensed at a flow rate of 0.6 mL/min.
This solution was loaded into a syringe, mounted into a programmable syringe pump Aladdin-10000, World PreciSarasotatruments, Sarasota, FL), and dispensed at a flow rate of 1 mL/h.
