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The lysis buffer was dispensed using an 8-channel LiHa pipetting head.
After incubation for 30 min at 37°C, cells were then stimulated with 150 nM PDGF (Sigma Aldrich) dispensed using an ECHO 555.
Cell solutions were dispensed using an automated liquid handling device, and cells exposed to drug (at a concentration of 10 μℳ) or control (0.1% DMSO) in growth media for 5 days, 24 h after seeding.
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HEK 293 cells stably expressing hCHT or hCHT LV-AA or control cells were assessed for basal and choline-induced changes in membrane depolarization as previously described by plating into 384-well, black walled, clear bottom poly- d-lysine coated plates (BioCoat, BD Biosciences, San Jose, CA, USA) at 20 000 cells in 20 μL/well, dispensed using a Thermo Electron Multidrop reagent dispenser.
Thus, acids were dispensed using a graduated cylinder instead of an acid dispenser.
Pancake batter dispensed using a patent-pending process similar to how Cheez Whiz is dispensed from an aerosol can? Patent pending?! It's gotta be good!
The HepG2 cell line was dispensed using a 70 µm piezoelectric nozzle.
For each well, 80 μl of crystallization solution was dispensed using a Thermo Scientific Matrix Hydra II robot.
The solution was loaded into a 5 mL plastic syringe with a 23 1/2-gauge needle attached and dispensed using a syringe pump.
Quadruplicate replicates of each DNA sample (5-10 ng/reaction) were dispensed using a liquid handling robot (EpMotion 5070, Eppendorf, Hamburg, Germany) into duplicate plates and dried down.
Drops of 200 nl SitA protein (with or without metal ions)+200 nl crystallization reservoir solution were dispensed using a Crystal Gryphon robot (Art Robbins Instruments).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com