Exact(8)
HEK 293 cells stably expressing hCHT or hCHT LV-AA or control cells were assessed for basal and choline-induced changes in membrane depolarization as previously described by plating into 384-well, black walled, clear bottom poly- d-lysine coated plates (BioCoat, BD Biosciences, San Jose, CA, USA) at 20 000 cells in 20 μL/well, dispensed using a Thermo Electron Multidrop reagent dispenser.
Thus, acids were dispensed using a graduated cylinder instead of an acid dispenser.
Pancake batter dispensed using a patent-pending process similar to how Cheez Whiz is dispensed from an aerosol can? Patent pending?! It's gotta be good!
The HepG2 cell line was dispensed using a 70 µm piezoelectric nozzle.
For each well, 80 μl of crystallization solution was dispensed using a Thermo Scientific Matrix Hydra II robot.
Quadruplicate replicates of each DNA sample (5-10 ng/reaction) were dispensed using a liquid handling robot (EpMotion 5070, Eppendorf, Hamburg, Germany) into duplicate plates and dried down.
The solution was loaded into a 5 mL plastic syringe with a 23 1/2-gauge needle attached and dispensed using a syringe pump.
Drops of 200 nl SitA protein (with or without metal ions)+200 nl crystallization reservoir solution were dispensed using a Crystal Gryphon robot (Art Robbins Instruments).
Related(1)
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