Exact(6)
The mixture was equally dispensed into two 1.5-ml centrifuge tubes.
The biofilm formation was carried out using the P63 strain of Pseudomonas aeruginosa and the M63 minimal medium [M63 salt 12 g/l KH2PO4, 28 g/l K2HPO4, 8 g/l (NH4) SO4, 1 mM MgSO4, 0.5%] 150 μl of M63 liquid medium was dispensed into two 12-well plates, and the seed culture of the clinical cultures cultured for 24 h was inoculated at 2% and cultured at 30 °C for 72 h.
The pellets of cells and porphyrin were resuspended in 1 mL medium and 4 × 100 μL of each concentration was dispensed into two 96‐well plates.
Approximately 8 ~ 10 g (mL) of stool was collected and then dispensed into two containers: a tube with Cary-Blair (C-B) culture medium for bacteria testing and a sterile box for virus testing.
Blood was extracted (5 ml), centrifuged to collect the supernatant, dispensed into two sterile tubes, sealed with sealing glue, and placed in a freezer at -20°C until used.
The cell and agarose suspensions were mixed without aerosolizing by aspirating and expelling with a 200-μL pipeter and were immediately dispensed into two wells of a 100-μL disposable plug mold (Bio-Rad Laboratories, HerCAles, Candallowedowed to solidify at ambient temperature.
Similar(2)
The mixture was stirred with a magnetic stirrer, dispensed into four 250 ml serum bottles and centrifuged at 3400 rpm and 25°C for 20 min using a Centra-GP8R centrifuge (Thermo IEC, Needham Heights, MA, USA), rotor 216-A.
50 µL per well of this suspension (20,000 cells per well) is dispensed into four microplates containing 367 biochemicals that could potentially be metabolized and provide energy for cells.
Related(1)
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