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Handkerchiefs are a necessity in Japan, where public restrooms even in chic restaurants or departmental stores do not dispense paper towels.
Dispense paper.
Culture containers were initially sealed with 4 dispense paper and parafilm layers.
In the present study also a combination of foil and dispense paper was found to be a favorable combination for callus proliferation in S. involucrata.
In the present study, a new approach of combining the foil and dispense paper was used for the G. scabra cultures.
Each culture flask containing 100 ml medium was closed with 4 layers of dispense paper (DP4) and an additional layer of parafilm which was removed after 2 weeks.
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3 layers of dispense papers (3DP).
Dispense papers had an additional layer of parafilm which was removed after 2 weeks.
While, in case of sealing of culture tubes with 3 layers of dispense papers, the callii were friable and fairer.
** AF2 = Culture container closed with 2 layers of Aluminum foil; DP4 = Culture container sealed with 4 dispense papers.
Sealing of culture vessels with aluminium foil or dispense papers had an influence on the quality and quantity of callus induced (Fig. 2).
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