These variable or dispensable steps may represent possible alternative pathways in different organisms and/or in different ecological niches, as has been previously suggested [ 10, 28].
A retrospective analysis of our protocol indicates that two dispensable steps, both involving gel electrophoresis for size selecting the chromPET library, are the most likely source for this contamination because all three patient libraries were processed simultaneously on the same gel.
The inference to generality is a dispensable middle step.
In addition, since the highly virulent strains under investigation are unlikely to produce other pili, our results suggest that pili might be dispensable for critical steps of the S. suis pathogenesis of infection.
We found that this step was dispensable and we omitted it for single or few (<10 cell) samples where contamination by genomic DNA was not a significant issue.
Analyses of reprogramming in Nanog-deficient cells show that it is fully dispensable for the initial steps: loss of differentiated characteristics and creation of a pre-pluripotent state.
Of note, H2A.X was shown to be dispensable for the initial steps of DDR as the recruitment of downstream factors in DSB signaling cascade was remained unaffected in cells lacking H2A.X as well as in H2A.X-deficient cells reconstituted with H2A.X version carrying S139A or S139E substitutions.
Evansia also possesses a reduced TCA cycle, able to produce all necessary metabolites, with HisC producing a bypass from oxalacetate to 2-oxoglutarate and making dispensable the three first steps of the cycle (encoded by gltA, acnAB, and icd) as it occurs in some Blattabacterium strains (González-Domenech et al. 2012).
Whereas the C-terminal region of MeCP2 is dispensable for the two first steps, it is apparently required for maximal compaction of the chromatin since the R294X mutation abolishes bridging between nucleosomes [60], [61].
In yeast, DOHH knockout causes only a very mild growth phenotype (Park et al., 2006; Weir and Yaffe, 2004), implying that the second step of hypusination is dispensable for the essential function of eIF5A in this organism.
