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Nevertheless, their viable phenotype also indicates that the Gab1/p85 interaction is, in comparison to the interactions of Gab1 with c-Met, Grb2 and SHP2, a relatively dispensable interaction during mouse development.
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The four-glycine motifs are dispensable for interaction of Mic10 with other MICOS subunits but are crucial for the formation of large Mic10 oligomers.
We found that while the TRAF6 RING finger domain is required for activation of TAK1, it is dispensable for interaction between TRAF6 and the TAK1-TAB1-TAB2 TAK1-TAB1-TAB2 TAK1-TAB1-TAB2
We found that while the TRAF6 RING domain is required for activation of TAK1, it is dispensable for interaction between TRAF6 and the TAK1-TAB1-TAB2 TAK1-TAB1-TAB2 TAK1-TAB1-TAB2
The C561 G566/C572 A577 sequences lack obvious complementarity to pre-rRNA sequences and they are dispensable for interaction with 35S pre-rRNA.
Furthermore, using different Rab5 truncation variants, we demonstrated that the N-terminus (residues 1−15) and C-terminus (residues 185−215) of Rab5 are dispensable for interaction with APPL1.
If the latter suggestion is correct, and NTE is dispensable for interactions, than co-expressed in yeast ΔNTE-NubG and ΔNTE-CubPLV constructs might interact since both would be in the same subcellular compartment.
However, the C71S mutant still keeps the partial activity of PTEN relative to its wild-type form 30. Here, we showed that the PTEN C71S mutant still interacted with AIF (Fig EV2N), suggesting that the cysteine is dispensable for PTEN AIF interaction.
These results suggest that BiP interacts with Ire1 region II IV in cells and that region V is dispensable for this interaction to occur, reinforcing the previous in vitro analysis.
Thus, the SH3 domain is apparently dispensable for the interaction between pyrin and PSTPIP1.
Our data reveal which domains of CHIP and LRRK2 are required or dispensable for this interaction.
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