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The problem with American politics is less that incumbents are being upset than that they usually cannot be dislodged with a crowbar.
Adherent cells were dislodged with trypsin/ethylenediaminetetraacetic acid (0.02%: 0.05%, w/v).
At 6, 12, 24, 36, and 48 h p.i., the tissue culture medium was aspirated from each well, and the cells were overlaid with an equal amount of SPG containing protease inhibitors, dislodged with a cell scraper, and harvested.
Cells were then washed three times with PBS, dislodged with 0.5 mM EDTA and dispersed into homogeneous single-cell suspensions in serum-free and phenol red-free αMEM to a concentration of 105 cells/300 µl.
At various time points post infection, the cells were preloaded with 1.5 nM DiOC6 in c-DMEM incubated at 37°C for 20 min, washed and fixed with 1% paraformaldehyde at 25°C for 20 min. The cells were then dislodged with a rubber policeman, pelleted at 500 x g, washed, and resuspended in PBS containing 1% paraformaldehyde.
Cells were dislodged with trypsin/EDTA in modified Puck's saline (Invitrogen).
Similar(44)
Those kind of memories can lead to scar tissue that can dislodge with the lightest scratch.
Her body is now marred with ingrown hairs that she attempts to dislodge with tweezers and needles, causing further damage to her skin.
Gently swish the silk flowers in the water, allowing the dirt and dust to dislodge with ease.
" 'Close to'!" Callista said, sitting up in bed so abruptly that a single strand of her hair dislodged itself with a crack and fell over her forehead.
This week, as Trimble compares the 15percentnt who supported the stalking horse that dislodged Molyneaux with the Reverend Martin Smyth's 43 per cent, it is perhaps even more unclear.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com