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At day 28, the animals were sacrificed and hemorrhagic transformation, subarachnoidal bleeding, or dislocation of the brain kit was excluded by histological examination.
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After rats were euthanized with isoflurane and cervical dislocation, specimens of the brain stem tissues rapidly flash frozen in liquid nitrogen were stored at −80 °C.
After cervical dislocation of the mice, the brains were carefully dissected, immediately frozen in dry ice cooled 2-methylbutane and stored at −80°C until use.
In brief, mice were killed via dislocation of the neck and decapitation; brain stems were rapidly dissected on wet ice and snap frozen on dry ice.
Two days later, the mouse was killed by cervical dislocation and the brain was excised.
The animal was then sacrificed by cervical dislocation and the brain was removed.
A subset of P301SBE-infused mice (2.5 months post-infusion) were killed by cervical dislocation followed by rapid removal of the brain and immediate dissection (as shown in Supplementary Figure 3); individual brain segments were snap frozen and stored at −80 °C.
24 For preparation of myelin membrane, adult mice were anesthetized with CO2 and killed by cervical dislocation followed by collection of the brains.
After the 31-day experiment, all rats were sacrificed by cervical dislocation and the brains were excised for further investigation of biochemical estimations.
Following cervical dislocation, the brains of newborn (1 to 2 day old) Wistar rats were removed.
Fifteen minutes after completion of the last training session, all experimental and control rats were simultaneously killed by cervical dislocation and the brains were immediately removed.
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