Neomycin resulted in slower FRAP recovery of WT α-actinin-2 (≈40 ± 4 s), similar to the PIP2 mutant, supporting the notion that α-actinin-2 Z-disk dynamics are strongly dependent on PIP2 regulation, in agreement with the Z-disk localization of PIP2.
Then the images are transmitted to be analyzed, including localization of optic disk and macular, vessel segmentation, detection of lesions, and grading of DR. We use a multi-scale line operator to improve accuracy in segmenting small-scale vessels, a binary mask and image restoration to reduce the effect of the existence of the vessels on optic disk localization.
Our observations are in accordance with a recent study in which localization of TRPC3 to the intercalated disks and intracellular membranes of ventricular myocytes was shown.
Localization of the GAL locus was scored by spinning disk confocal microscope as described (Berger et al., 2008).
In both normal and atrophied skeletal muscles, the localization of mDia1 was mainly confined to the Z-disk, and cytoplasmic Rb observed in atrophied muscles colocalized with mDia1.
After fixation with 3.7% paraformaldehyde for 20 min at room temperature, subcellular localization of EYFP fusion proteins was analyzed with a Perkin Elmer spinning disk confocal microscope Ultraview RS system.
This involves the precise localization of sub-surface musculoskeletal structures such as growth plates, intervertebral disks and vertebral bodies.
To assess the architectural organization of cell colonies in 3D culture, expression and localization of total ezrin, Y477F-ezrin (VSVGtagged)and F-actin were examined using confocal spinning disk microscopy and deconvolution image analysis.
We also performed spinning disk microscopy on HeLa cells treated with PI3K inhibitors and infected with B5-GFP to more clearly resolve membrane localization of late proteins.
Subcellular localization of Cy3-labeled CD22ΔE12-siRNA CD22ΔE12-siRNAhe PVLGB-8 basedeliverednanopartinles was examined by immunofluorescence and spinning disk confocal microscopy using previously described procedures for slide preparathen and imaging (Uckun et al., 2012).
Localization of mobile node.
