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Negative controls were prepared using a disk impregnated with the same solvent as that used to dissolve the plant oils.
Evidence of prior antimicrobial treatment was garnered through an assay in which a disk impregnated with the patient's CSF was placed on an agar plate seeded with S. aureus ATCC 25923.
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Antibiotic sensitivities were assayed using Kirby-Bauer disks impregnated with ceftriaxone.
Disks impregnated with solvents used for solubilization of extracts were used as negative control.
[ 26, 29– 32] Nylon disks impregnated with either basic FGF or VEGF were implanted into one cornea of rats and then treated with IL-1 (anakinra) or TNFα (soluble receptors) blockade for 7 days.
*TCBS, thiosulfate-citrate-bile salts-sucrose; O129, disks impregnated with 2,4-diamino-6,7di-iso-propylpteridine 2,4-diamino-6,7di-iso-propylpteridine 2,4-diamino-6,7di-iso-propylpteridine 2,4-diamino-6,7di-iso-propylpteridine 2,4-diamino-6,7di-iso-propylpteridinederived according to criteria from [ 4 ]).
Disks impregnated with the following drugs were used: penicillin (10 μg), oxacillin (1 μg), cefoxitin (30 μg), erythromycin (15 μg), cephalothin (30 μg), gentamicin (30 μg), and rifampicin (30 μg).
We previously determined the sensitivity of C. albicans strains to fluconazole by the Kirby-Bauer disk diffusion susceptibility test [ 21] using 6 mm filter paper disks impregnated with 10 μg of fluconazole obtained from DHN (Cracow, Poland) and YNB agar (Yeast Nitrogen Base-Difco 0.5%, glucose 3%, agar 1.8%, pH = 7) also obtained from DHN (Cracow, Poland).
Disc impregnated with DMSO were used as control.
A filter-paper disk was impregnated with 20 ul from the resulting suspension.
39 Recently, paper disks were impregnated with sera from patients recruited to a study of community-acquired pneumonia/bacteremia in NE Thailand and tested against lawns of Staphylococcus aureus 24% of patients had evidence of anti- S. aureus activity in their sera.
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