Using a titanium disk in combination with a gold ring, potential sweep experiments using the RRD/EQCM were carried out on the anodization of titanium in sulfuric acid.
The cefotaxime (CTX) and ceftazidime (CAZ) disks in combination with clavulanate (CLA) were performed and interpreted by CLSI criteria for ESBL screening and disk confirmation tests [ 7].
An isolate was classified as having an ESBL-producer phenotype if the inhibition zone differed by ≥ 5 mm between at least one of the standard antibiotic disks and its corresponding clavulanate combination disk.
Several phenotypic detection tests, based on the synergy between a third-generation cephalosporin and clavulanate, have been designed: the double-disk synergy test (DDST, ESBL Etestss, and the combination disk method.
This paper presents a generalized formulation for the in-plane modal characteristics of circular annular disks under combinations of all possible classical boundary conditions.
An increase of >5 mm in the inhibition zone of the combination disk when compared to that of the cephalosporin disk alone demonstrated ESBL production.
ESBLs detection was done by the combination disk method according to the CLSI guidelines [ 5], using disks containing ceftriaxone or ceftazidime with clavulanic acid (30/10 μg) and disks containing ceftazidime (30 μg) or ceftriaxone (30 μg) alone.
Another important property of RDDs is that they can reside in the memory, disk or in combination.
Each isolate generating a non-determinate (ND) result with this gradient test was retested using the combination disk test (CDT) as described by Carter Carterr et al. 2000).
All isolates were assessed for ESBL-production by the E-test method and those giving undetermined ESBL status were re-tested using the combination disk test.
