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The catalytic core domain of integrase (IN CCD) is capable of catalyzing disintegration reaction.
Results showed that the disintegration substrate could be recognized and catalyzed by IN CCD, and the disintegration reaction can be monitored according to the increase of fluorescent signal.
In this work, a hairpin-shaped disintegration substrate was designed and validated by enzyme-linked immunosorbent assay; a molecular beacon-based assay was developed for disintegration reaction of IN CCD.
Palindrome cleavage also differs markedly in this respect from the disintegration reaction that can be performed by CC, ΔC or ΔN truncated proteins only in the presence of Mn2+, or by the full-length IN with a large preference for Mn2+ over Mg2+ [24], [24].
Sequence specificity of the disintegration reaction at the −1 position.
(A ) E. coli Cas1 also catalyses an efficient metal dependent disintegration reaction.
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The aim of this paper is to simulate the chemical transformations of the engineered barrier and chemical-elements diffusion impact resulting from the temperature increase (disintegration reactions of wastes), the presence of fluids coming from the geological barrier, and from the liberation of iron (degradation of the containers).
It remains unclear whether EcoCas1 prefers the disintegration reactions at site 1 or site 2.
The disintegration reactions catalysed by diverse integrases have proven a powerful model system for the understanding of the mechanism of integration.
We proceeded to investigate the sequence dependence of the nucleotide at the 3′-end of the attacking DNA strand (the −1 position) in the disintegration reactions.
Furthermore, Nunez et al. demonstrated that 33 bp double-stranded DNA fragments can be integrated into CRISPR repeats by the Cas1 and Cas2 proteins and both the forward (integration) and reverse (disintegration) reactions were observed and characterized.
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