Early trochophores developed in the jelly mass by 4 6 h, 3-chaetiger metatrochophores after 48 h, and the jelly mass had totally disintegrated by 72 h, releasing the lecithotrophic larvae.
In tumor tissue, preexisting blood vessels within the tumor graft disintegrated by 24 h after implantation.
In contrast, FM ipsilateral subchondral plates deteriorated dramatically, and trabecular bone was almost completely disintegrated by 20 weeks.
However, the spinach leaf disks stored at 22°C in constant darkness disintegrated by 6 days and were therefore unable to be further analyzed.
The fact that the epidermis was disintegrating by E18 and thus definitely not normal skin further excludes reversion as its basis.
After incubation cells were disintegrated by adding 150 μL 2 mM Z-sarcosyl-buffer, the OD600 measured and the microtiter plate incubated at 30°C for 20 min for the complete disintegration.
The cell pellet was resuspended in 500 μl PBS containing 10 mM NaCNBH3 and disintegrated by pulsed ultrasonication (15 pulses for 1 s) at 4 °C.
The rigid pellicles of PA14 could not be disintegrated by mild procedures [13].
Cells were centrifuged at 4000 rpm for 5 minutes, washed with buffer A [50 mM Tris/HCl, 1 mM EDTA, pH 7.5] and disintegrated by vortexing with zircon beads (diameter 0.7 μm, Biospec, Bartlesville, OK, USA) 2 1 in buffer A with plant protease inhibitors (Sigma-Aldrich, Prague, Czech Republic) for 6 minutes.
Since the viral glycoproteins E1 and E2 can be separated from each other under non-reducing conditions, virus samples were first disintegrated by incubation for 20 minutes at 56°C in Laemmli sample buffer containing no 2-mercaptoethanol.
