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Between them they have won nine singles crowns at the All England Club and any champion to lift the Venus Rosewater Dish in the absence of the American siblings would be left with an asterisk next to their name on the honours board.
One section of stem was placed in each petri dish, in the absence of any media and a sterile scalpel used to score the surface.
hES cells were harvested by using dispase or trypsin-EDTA, plated on non-tissue culture treated dishes (approximately 107 cells/ 10 cm dish) in the absence of Y27632, and grown in non-conditioned medium for 7 days.
ESCs were seeded onto a petri dish in the absence of a differentiation antagonist to form embryonic bodies (EBs).
Mature oocytes were obtained from the oviduct and placed in a 35-mm diameter Petri dish in the absence of seawater.
Exponentially growing VA13, AT22, and EA.hy926 cells were plated at a density of 1.5 × 10 cells/100 mm tissue culture dish in the absence or presence of lipoproteins in normal growth medium.
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On the 5th day, EBs were collected and plated in complete α-modified Eagle's minimal essential medium in tissue culture dishes in the absence of inducers (NI) until day 14.
Undifferentiated cells were trypsinized and induced to form EBs by allowing them to aggregate in suspension culture by growing them in nonadherent plastic petri bacterial dishes in the absence of bFGF as previously described [12], [18].
P19 EC cells were aggregated for 4 days in the presence of DMSO, with or without RA, and then plated into tissue culture dishes in the absence of drug.
Tissue was then either placed on plates containing Murashige and Skoog (MS) agar [17] +1.5% sucrose with or without 200 µM acetosyringone, or was placed in petri dishes in the absence of any media.
For Listeria growth/killing assays, macrophages were seeded in non-tissue culture treated dishes in the absence of M-CSF.
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