Postnuclear supernatant (PNS) was prepared by solubilization of cells in 400 µl/3,5 cm dish (or 800 µl/6 cm dish) ice-cold 2% CHAPS (Anatrace) in HEPES-buffered saline (HBS), pH 6.8, containing 20 mM N-ethylmaleimide and protease inhibitors.
Postnuclear supernatants (PNS) were prepared by solubilization of cells in 400 µl/3,5 cm dish (or 800 µl/6 cm dish) ice-cold 2% CHAPS (Anatrace) in HEPES-buffered saline (HBS), pH 6.8 containing 20 mM N-ethylmaleimide and protease inhibitors.
We walked home and Michael sat at his computer screen, presumably checking email while I dished ice cream into two bowls.
There are organic lamb kabobs, pork a dozen different ways, fried clams, shrimp and haddock, egg sandwiches, dozens of mouth watering vegetarian dishes, ice cream, baked goods, smoothies, carrot juice, gluten-free everything, tacos, crabmeat rolls, baked potatoes, home fries, wraps, tempura, baked beans, cookies, chai and more!
The characteristic anise odour of F. vulgare, which is due to its essential oil, makes it an excellent flavoring agent in baked goods, meat and fish dishes, ice-cream, and alcoholic beverages.
The striatum, and SN were dissected out on a chilled glass dish on ice.
Reporters had a dish of Ice Lean, which is ice cream with half the usual number of calories.
Fill Petri dish with ice and keep on ice.
The flow-through was collected in a 60-mm glass petri dish on ice, and then applied to a 40 um filter.
Walt tenderly fed her a dish of ice cream and a dish of pudding.
