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disgregation
noun
Separation; scattering.
Exact(4)
Mechanical disgregation: small pieces of tissues were disgregated using forceps and scrapels and then filtered with medium.
In order to demonstrate that the SVZ harbors cells which are sensitive to extracellular ATP, primary neurospheres obtained from SVZ disgregation were loaded with the fluorescent calcium indicator Fura-2 AM.
This is compatible with the observed intererence of P. intermedia adhesion to gingival cells [ 8, 41], biofilm formation and disgregation [ 8].
Tumor samples were immediately processed by mechanical disgregation and plated on gelatin coated flasks (Sigma, Saint Louis, MO, USA) in MCDB (Sigma), 2 mM L-glutamine (Gibco Ltd, Paisley, Scotland), recombinant human bFGF (10 ng/ml, Peprotech, London, UK), recombinant human EGF (20 ng/ml, Peprotech), 15 μg/ml insulin, 2 μg/ml heparin and 2% FBS (Euroclone, Devon UK).
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