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In this study, we implemented LD-Spline, a database routine that defines the genomic boundaries a particular SNP represents using linkage disequilibrium statistics from the International HapMap Project.
The linkage disequilibrium level among markers (SNPs and SSRs) identified within the apple cultivar collection, was calculated and visualised using Haploview 4.2, a software package designed for linkage disequilibrium statistics and haplotype block inference from genotype data [ 45].
Linkage disequilibrium statistics and haplotypes analysis, including haplotypes frequency estimation, as well as an analysis of association between haplotypes and PE, were made using SNPStats software http://bioinfo.iconcologia.net/index.php?module=Snpstats.
In this work, we introduce LD-Spline, an efficient database procedure for establishing genomic regions that a SNP potentially represents by mining linkage disequilibrium statistics available from the International Hapmap Project.
This model has been used primarily to evaluate the influence of population subdivision and admixture on the performance of the transmission-disequilibrium test (Ewens and Spielman 1995) and to examine linkage disequilibrium statistics (Guo et al. 2005).
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The possibility of intragenic recombination, which would prohibit the use of the respective loci for phylogenetic analysis, was tested by linkage disequilibrium based statistics as implemented in DnaSP 4.50.3 [46].
Neutral evolution was tested by linkage disequilibrium based statistics and Tajima's test as implemented in DnaSP 4.50.3 (Rozas et al., 2003).
The mDPCoA could be associated with other tools to study population structure, including the AMOVA, which forms the basis of the DPCoA, Linkage Disequilibrium (LD) statistics, and also recent approaches such as STRUCTURE or CLONAL FRAME.
Haplotype blocks were constructed using Haploview, version 4.2 (Broad Institute of MIT and Harvard, Cambridge, MA, USA), a software package that provides computation of linkage disequilibrium (LD) statistics and population haplotype patterns from genotype data [ 16].
Informative SNPs were identified both in the centromere of chromosome 2 and in the flanking DNA and in the TER1 gene and in the flanking DNA and used to estimate linkage disequilibrium (LD) statistics using DNAsp (Table 5).
Haplotype blocks (bins) were constructed using LDselect (Christopher S. Carlson and Deborah A. Nickerson, Seattle, Washington, USA), a software package that provides computation of linkage disequilibrium (LD) statistics and population haplotype patterns from genotype data [ 45].
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