Analysis of MsbA conformational dynamics reported previously (Dong et al., 2005; Borbat et al., 2007; Zou and Mchaourab, 2009) and extended here is entirely consistent with a model of turnover that invokes engaged and disengaged NBD states, symmetric NBS conformations, and tight structural coupling between the NBDs and the TMDs.
By now, according to popular wisdom, I should be disengaged and furious with the state of education.
Over all, boredom is a state of "being disengaged from one's environment," he said, and it reflects a passive relationship to one's work.
According to Eurofound, as many as 14 million young Europeans are out of work and disengaged, costing European Union member states an estimated €153 billion (about $206 billion) a year in welfare benefits and lost production.
In the absence of nucleotide or substrates, hereafter referred to as the apo state, BmrCD NBDs are disengaged as evident from distances between spin labels that are larger than those predicted on the basis of the nucleotide-bound TM287/288 crystal structure and the BmrCD homology model.
For instance, the myristic acid group is more disengaged from the enzyme in an apo state than in the ligand bound states based on very little or no electron density for the myristic acid group for chain A and chain B, respectively.
We report the h44-h45 interface in varying states, i.e. engaged, disengaged and in equilibrium.
We have seen states who were once disengaged suddenly competing against their neighbours to meet the targets first.
Indeed, Leon's view of the adults who surround him (in their varying disengaged, awkward, angry, drunk, or lonely states) is perceptive, and, on occasion, startlingly funny.
A lost generation of 14 million out-of-work and disengaged young Europeans is costing member states a total of €153bn (£124bn) a year – 1.2% of the EU's gross domestic product – the largest study of the young unemployed has concluded.
