Indicator mice developed clinical prion disease after inoculation with Rocky Mountain Laboratory mouse-adapted prion strain (RML) at the three dilutions tested, but not when they were inoculated with mock control, as expected.
Thus, the 0ΔNLS mutation in the ICP0 gene allowed a mild HSV-2 infection to be established in ICR mice that did not produce any overt symptoms of disease following inoculation of the eyes, nose, vagina, or footpads.
Successful experimental transmission of the disease through inoculation with fresh scrapings from DD lesions was described in 1996 [ 12].
For disease assays, inoculation of B. cinerea was performed using spore suspension at spore density of 1 × 10 spores/mL according to previously reported procedure [ 74].
However, subsequent investigations questioned whether these treponemes were viable, since the transfer of material from the lymph nodes of some treated syphilis patients produced no detectable disease upon inoculation into rabbits [ 50].
Of the 4 sCJD strains, subtype VV2 showed the greatest frequency of clinical (13/16), vacuolar (16/16), and pathologic (15/15) signs of prion disease following inoculation into HuVV mice (Table 3) (3 ).
All Microbotryum genotypes produced by hybrid backcrossing caused anther-smut disease following inoculation of the natural host S. latifolia and the artificial host S. colorata[ 33], allowing assessment of the influence of native versus novel host environment (extrinsic factors) and mating type region (intrinsic factors).
Depending on the shelter, foster care involves permanent placement of older or chronically ill animals, temporary housing for those recovering from illness or injury and temporary housing for newborn puppies and kittens that are susceptible to communicable diseases before inoculation.
Clinical disease post-inoculation with S. Choleraesuis was observed in the animals in this study, and described previously [ 8].
