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Ten discs were selected at random from the 15 discs obtained from each plot.
Bark thickness was one of the many attributes measured from 1063 discs obtained from the sample trees.
Discs, obtained from Oxoid UK, were used at the following strengths: ampicillin, 10 µg; amoxicillin-clavulanic acid, 30 µg; oxytetracycline, 30 µg; ceftiofur, 30 µg; enrofloxacin, 5 µg; streptomycin 10 µg; sulphonamide-trimethoprim, 25 µg.
The cartilage discs obtained from different locations were randomly distributed to the different experimental groups.
Asymptomatic normal and degenerate discs obtained from donors at PM had no documented clinical history of LBP.
NP was dissected from the centre of the three uppermost bovine caudal discs obtained from young adult cattle (n = 15, ages 18 to 32 months) within 1 hour of death with permission from a local abattoir.
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Standard Neomycin (NEO30) discs were obtained from Oxoid Ltd., England.
Antibiotic discs were obtained from MAST Group (UK).
Antibiotic discs were obtained from Oxoid (Nepean, Ontario) and stored at 4 °C until use.
Paper discs were obtained from the filter paper by using a hand punch (M.C. Mieth Manufacturing Inc).
Antibiotic sensitivity discs were obtained from Biodisc AB (Solna, Sweden) and the anaerobic pouch system (AnaeroGen compact) from Thermo Fisher Scientific (Oxoid, Basingstoke, UK).
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