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The cultured cells were loaded on iron oxide-silica nanocomposite discs at a seeding density of 5 × 10 cells/cm in 24-well plates.
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After harvesting, the cells were resuspended in the medium, centrifuged at 200×g for 5 min and plated at a seeding density of 5 × 104 cells per cm2 of the tissue culture 12-well plates (Sigma-Aldrich, TPP ®, Steinheim, Germany) containing substrate (Si, Si + Au, Si + Au + ZnO urchin2, Si + Au + PVDF-HFP, Si + Au + ZnO urchin2 + PVDF-HFP and a plain Zn disc).
Burning discs at a slower recording speed or using different brands of discs may also help solve the problem.
The Samsung SM-352 drive can record CD-R discs at a speed of 52x and record data to the reusable CD-RW discs at 24x.
Warwick Davis on Desert Island Discs at a glance.
It holds up to five discs at a time, and comes with a rapid-reload built-in disc clip.
Does not include online viewing), GBP9.99 a month for two discs at a time (limit of three per month plus 4 hours online viewing), GBP14.99 a month for two discs at a time (Unlimited discs by post and online viewing) and GBP18.99 for three discs at a time (Unlimited discs by post and online viewing), all with free postage and no late fees.
For picking single seeds, the planter disc had a seed hole of 2·5 mm in diameter.
In the early 1970's, he created disc golf, which involves throwing a Frisbee-like disc at a metal cage.
This little devil shoots one disc at a time and comes with three discs.
The extracts were incorporated in a 5 mm sterile disc at a concentration of 500 μg /disc.
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