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To date a large number of organisms have been typed by MLST, which proved to be a highly discriminatory technique [29] [31].
To date, a large number of organisms have been typed by MLST, which proved to be a highly discriminatory technique [ 29].
There is a growing research showing that mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) is the most discriminatory technique to genotype M. bovis isolates [ 1, 13, 14].
In this study, we investigated the relationship between clinical and environmental isolates of V. cholerae on the basis of random interrogation of 2.08% of the genome by AFLP, a highly discriminatory technique, using six primer combinations.
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This is not easily determined using spoligotyping alone and would therefore require more discriminatory techniques such as MIRU and restriction fragment length polymorphism (RFLP) to confirm this.
Cryptic species or subpopulations that exhibit divergent behaviors (7 ) may be responsible for maintaining malaria parasite transmission, and without adequate discriminatory techniques, these vectors may be misidentified and their key behavioral differences overlooked.
We then used analysis of variance to compare the different discriminatory techniques, Pearson's correlation coefficient to examine the association between test scores and participants' perceived discriminatory ability, and repeated measures analysis of variance to examine the effect of figure position (front, back, or mixed) on test scores.
In case of strains showing identity at phenotype and virulence profile evaluated, a high discriminatory power technique, PFGE, was proposed to confirm the relation between isolates.
Epidemiologic studies to identify possible sources of legionellosis require careful investigation, including sensitive and discriminatory subtyping techniques to identify similarities and differences between possibly related strains.
Studies on clustering rates for other African countries are rare and diverse both in methodology and results; only a few of them have used highly discriminatory typing techniques.
The following technical prerequisites permitted this large-scale investigation: free availability of the cluster detection software SaTScan, implementation of an automatic data transfer between our database and SaTScan, availability of data regarding time and place of occurrence of IMD, and state-of-the-art highly discriminatory finetyping techniques for the infectious agent in question.
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