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Also, this suggests that if the "markers" for discriminating cells with enhanced tumourigenic potential will be identified in melanoma in the future, they will most likely mark a relatively large cell subpopulation.
Although in this case we only report results of an algorithm locating and counting cells without regard to size or shape, the CellC algorithm or one similar could be easily used to report morphometric parameters that could enable an approximate complete blood count (CBC), discriminating cells into several blood cell classes.
PC-LDA results were shown for discriminating cells between Groups N- and N + at each time point.
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This method has stronger distinguishing ability, showing better performance in discriminating cell images whether belong to the same cell.
We then investigated key genomic features that were critical for discriminating cell cycle genes from non-cell cycle ones.
We conclude that the JAK2 V617F /PNA-FISH method displays high specificity and reliability in discriminating cell subpopulations harbouring the JAK2 V617F mutation.
We next assessed whether we could identify markers that could discriminate cells that had the potential to differentiate into fibrocytes.
In the following experiments, we used mainly γ-H2AX detection to discriminate cells with 32Hc induced response.
Several independent surviving clones were isolated and analyzed by RT-PCR to discriminate cells that were homozygous for the trapped allele (sik1flp/flp) over sik1wt/flp cells.
However, we did observe that β1-integrin expression might be useful to discriminate cells degrading ECM as single cells (SKOV3 and UPN251) from those that induce ECM rearrangement (e.g., OVCAR10).
This indicates that in highly aggressive melanoma, the functional "marker" ALDH does not discriminate cells with enhanced biological aggressiveness, and ALDH+ subpopulation does not play an exclusive role in tumour initiation and/or in low response to therapy.
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