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BAC-DNA was extracted with a Plasmid Midi kit (QIAGEN GmbH, Hilden, Germany) and labeled with a fluorochrome using a Nick Translation System (Invitrogen, Carlsbad, CA, USA), with one of four fluorochromes, which were discriminated with different filter sets.
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In another study that investigated whether 4-day-old infants discriminated syllables with different numbers of consonants-vowels, infants discriminated 2 vs. 3 items but not 4 vs. 6 items [68].
Different dilutions of IAA vs EB (10−4, 10−2 and 10) were also discriminated with similar speeds, though significantly different.
For discrimination validity we evaluated whether the scale and domains could discriminate those with different QOL: men with and without BPH, patients with BPH of different severity according to the IPSS (Total score has a range of 0 to 35: mild 0 7; moderate 8 19; and severe >20), and patients recruited from different settings inpatient, outpatient and community-based.
The test method also did not sufficiently discriminate formulations with different in vivo absorption rates.
However, specific tests for grip strength endurance using the gi can discriminate athletes with different experience and competitive levels in Brazilian jiu-jitsu.
While it is important from a biological and mechanistic perspective to characterize at best all the genes that are influenced by a given condition, from a clinical perspective, it might be more relevant to identify a limited set of gene expressions that could efficiently discriminate individuals with different risk profiles.
Expression levels of circulating miRNA in plasma can be used to discriminate patients with different outcomes.
In this study, we have used a different strategy, hypothesizing that the identification of distinct molecular tumor subtypes would likely discriminate patients with different clinical outcomes, as well.
Our benchmark results suggest that using complex computational models is less important than using highly discriminating features with different biological background.
The present study suggests that the degree of neck pain was gradually related to psychosocial distress and that different levels of neck pain might discriminate patients with different degrees of psychological distress.
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