Unfortunately, some miRNA quantification methods such as miRNA microarray cannot effectively discriminate the expression levels of pre-miRNAs from those of mature miRNAs [37], [38].
Owing to the high sequence identity (≈ 97%), it was impossible to discriminate the expression of cytoplasmic isoforms (ACT1A-D) and muscle-specific isoforms (ACT2A-C).
One of the limitations of the approach used in our study is that the E p/Ē t ratio does not discriminate the expression of the A genome homoeolog from that of the B genome.
One of the major advantages of using an oligo array of this nature is that it allows to clearly discriminate the expression levels in terms of transcript abundance of different gene family members, enzymatic isoforms and protein subunits.
PCR detection of both extracellular and cytoplasmic portions of ALK was carried out using different set of primers, in order to discriminate the expression of full-length ALK and truncated variants arising from chromosomal rearrangements, whereas western blot analysis was performed to determine endogenous protein expression and activity.
Satelli et al. [ 63] developed an anti-vimentin antibody able to discriminate the expression of cell-surface vimentin, mainly associated with cancer cells, from the intracellular vimentin expressed by most white blood cells in order to exclude bias to use this protein as a CTC marker.
We were also able to discriminate the expression profiles of individual gene family members, enzymatic isoforms and protein subunits and group them accordingly to their involvement in different functional activities relevant to seedling development and the cotyledonary functional transition in soybean.
Following differentiation, Sox17 regulates hemogenic endothelium (Clarke et al, 2013; Nakajima-Takagi et al, 2013) and its hematopoietic progenitors that can be further discriminated by the expression of CD43 or the pan-hematopoietic marker CD45 with a surface marker phenotype of lin−/CD34+/CD45+/CD38− (Rafii et al, 2013).
This involves not only perceiving and discriminating the emotional expression.
Primer sets were designed to discriminate the gene expression level of whole families, subfamilies or individual members.
