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More importantly, direct and indirect targets of the TF cannot be discriminated by expression alone.
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Following differentiation, Sox17 regulates hemogenic endothelium (Clarke et al, 2013; Nakajima-Takagi et al, 2013) and its hematopoietic progenitors that can be further discriminated by the expression of CD43 or the pan-hematopoietic marker CD45 with a surface marker phenotype of lin−/CD34+/CD45+/CD38− (Rafii et al, 2013).
Hepatic myofibroblasts are a heterogeneous population of cells characterized by expression on α-smooth muscle actin and might be discriminated by tissue localization, expression of different markers and response to stimuli (Fig. 1).
Germ cells and somatic cells were discriminated by their differential expression of vimentin [12] (vimentin is only expressed by somatic cells).
2 The B-1 lineage was originally defined based on expression of the Ly1/CD5 surface antigen, but these cells can be further discriminated by their differential expression of CD5 and CD11b/Mac1 into B-1a (CD19hiCD5hi) and B-1b (CD19hiCD5loCD11bhi) subsets.
Thus, the KIT-mutant GISTs of groups 1 and 3 could be discriminated by higher relative expression of CD34 and PDGFRA in group 3, and higher relative INSR expression in group 1.
Firstly, ER status was discriminated by fitting the bimodal expression of ESR1 to a mixed Gaussian model.
Different transcripts are discriminated by their 3′-most tags and expression levels estimated with higher accuracy than could be achieved by random RNA-Seq [ 52].
Today, these problems can be circumvented in well-annotated genomes because strand specific expression can be discriminated by the use of oligonucleotide capture probes.
Gene expression measurements were clearly discriminated by isolation method although the reproducibility was high for all methods (range ρ = 0.90-1.00).
Others expressed being discriminated by their HIV negative siblings.
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