Recently, we successfully used a double tandem hybridization strategy in the genesensor chip system to discriminate point mutations in codon 634 of RET oncogene in individuals affected by medullary thyroid carcinoma [ 23].
The following linear model: Yijk = μ + Ti + Gj + (G*T ij + ϵijk, where Ti is the treatment effect (i = irrigated vs. non-irrigated), Gj the family effect (j = "V+" vs. "V-") and (Ti*Gj) is the interaction effect, was used, with P-values < 0.05 considered to identify significant discriminating points.
What feedback loops can potentially discriminate filaments pointing to the leading edge from filaments pointing backwards?
Item-level analyses revealed generally good distributional properties, inter-rater agreement, discriminating anchor points, and preliminary convergent and discriminant validity.
Using the MI-MB, we could readily discriminate the point mutation-contained p53 from the wild-type one.
We used a receiver-operating characteristic (ROC) curve, a plot of the sensitivity versus 1 – specificity, to display the trade-off between true positives and false positives across incremental choices of discriminating cutoff points for glucose disposal rate (9, 10).
The SNP detection is based on the ability to discriminate single point mutations and relies on DNA polymerase with proof reading activity, to extend a primer only when its 3'-end is perfectly complementary to the template [ 25].
Critics, although they'd deny discriminating, quickly point out she's the oldest active senator and will be 85 at the next election.
By contrast, the species were discriminated by point mutations observed at the trnL-F intergenic spacer region.
To search for the most discriminating cutoff point between early and late CBC, we modelled CBC risk according to the different cutoff times since the first BC.
