Briefly, quantitative analysis of vessel ID was based on measurements from the middle of discrete vessel segments in individual cine-radiogram frames using Image-J (v1.41, NIH, Bethesda, USA) for individual rats during each treatment period.
However, using SAM to resolve the individual mechanical contributions of discrete vessel sub-structures [18], we identified significant changes in acoustic wave speed that were localized to the inter-lamellar regions of the medial layer.
Compared to existing techniques, DWP-OCT provides some important features for SaO2 measurement including depth-resolved measurements and capability to measure SaO2 levels in discrete vessels.
Counting of discrete vessels was performed with a magnification of × 200, using a 25-point Chalkley grid as described by Hansen et al (2000a, 2000b).
This transport was initially driven by interstitial flow (i.e. no discrete lymphatic vessels could be visualized at 2 weeks); however, by 6 weeks honeycomb-like dermal lymphatics were noted in the transferred grafts (primarily at the distal margin) suggesting that the reformed lymphatic vessels are functional (Figure 1D).
Non-invasive depth-resolved measurement of hemoglobin oxygen saturation (SaO2) levels in discrete blood vessels may have implications for diagnosis and treatment of various pathologies.
Briefly, in an optical microscope (E400, Nikon Instruments, Melville, NY) hot-spot areas for CD105 expression in discrete blood vessels were initially identified by scanning the entire tumor at low power (× 50 and × 125).
Dye injected far from the heart tube returned to the heart via preferred flow channels, despite the absence of discrete return vessels (Fig. 6; supplementary material Movies 8, 9).
Salsbury et al (1970) found that the treated tumours were less hyperaemic on histological examination compared to controls, and instead of the network of poorly defined vascular channels, treated tumours contained only a few discrete blood vessels (Salsbury et al, 1970).
Attribute labels, as opposed to being discrete numbers as in vessel type labels or IMO number labels, are continuous and might be unique for each vessel.
However, an automated count of this image would result in a much higher number of objects, corresponding to each of the discrete segments of each vessel.
