These CpG-rich promoters showed discrete initiation patterns rather than broad regions of transcription initiation, as are typically seen for CpG-island promoters.
Early expression vectors used the U6 and H1 RNA polymerase III (pol III) promoters which use discrete initiation and termination marks [ 9, 10].
We have previously demonstrated that the movement parameters of rats at the wait-start and nose-poke events are highly consistent, and particularly that the nose-poke event is consistently preceded by a discrete initiation of movement on all trial types (Donnelly et al., 2014).
With the exception of the Christchurch Kurdish community, between six and eight discrete snowball initiation points were used with each of the other three groups, with a variety of people radiating out from each 'entry' point.
G2 arrest involves multiple discrete steps including initiation, maintenance, adaptation and release that occur through distinct mechanisms.
In MIRA, we focused on the clinical testing stage, and the broad domain of "acceptability" included an evaluation of user perspectives about the physical characteristics of product use (including comfort, ease of use, effect on sexual pleasure), as well as discrete use, female-initiation and partner support for use.
Fiber development spans four discrete, yet overlapping stages: initiation (-3 to 5 days post anthesis, dpa), elongation (3 to 21 dpa), secondary cell wall deposition (14 to 45 dpa) and maturation/dehydration (40 to 55 dpa) [ 4].
Time between translocations was the length of time between the initiation of two discrete movements of the cell rear.
We conclude that our method, and indeed any statistically-principled method for cryptic initiation analysis using discrete expression-based probes, can more readily identify sites of cryptic initiation in long genes, where more probes flank every putative site.
Replication initiation sites are discrete chromosomal loci defined by the binding of the ORC and the Mcm2-7 complex.
At the heart of the pX1.0 construct is an archetypical IncX1 replicon which encodes the replication initiation protein π, initiator of plasmid replication through a Cairns type (Θ type) mechanism at three discrete origins of replication (α, ß and γ), along with the Bis protein, required for replication initiation at the ß-origin.
