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One 96-well anion exchange resin plate was used to fractionate samples into six discrete fractions (pH 9 + flow through, pH 7, pH 5, pH 4, pH 3 and organic wash) as previously described by Koopmann et al. (2004).
The method assumes that the interface and the rest of the system can be modelled as a series of discrete fractions each with its own glass transition temperature.
As discrete fractions show different times of decomposition in the range of 20-50 years for particulate SOM and about 100 years or more for mineral associated SOM [15, 16, 18], this decrease is assumed to be a long-term one at the site of this investigation.
As the Microrotofor Cell generated discrete fractions according to protein pIs, it enabled the use of a variety of pH gradients in this study to optimize protein separation conditions.
Discrete fractions were collected and concentrated in IB and stored at −20°C in IB plus 20% glycerol.
Thymic DCs were divided into four populations with discrete fractions of both CD8α+Sirp-α− and Sirp-α+ DCs expressing Id2-GFP.
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Therefore, in order to detect a protein in the 1 ng per ml range in blood, a starting sample in the tens to hundreds of microlitres of blood would have to be efficiently captured and fractionated, to deliver 1-10 femol mol in a single discrete fraction within detection limits and in agreement with the sample sizes used in some of the studies cited here.
The combinatorial analysis of binding motifs in this discrete fraction of the genome will facilitate the definition of the transcriptional regulatory networks.
It is shown that the curve may be well approximated by summing the desorption curves of the individual, discrete size fractions, weighted by their volume fraction.
The method employs a multistep, retained pH gradient formed using adsorbed buffering species to produce a series of discrete effluent fractions.
This paper proposes a method to estimate the curvature of an interface represented implicitly by discrete volume fractions on an unstructured two-dimensional mesh.
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