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They transformed the quantitative expression levels into discrete expression patterns (expressed or unexpressed).
To overcome possible complications from 5′UTR secondary structures — due to the presence of multiple λCl binding sites within the same mRNA transcript — design IV, which comprised synthetic hybrid promoters of pBAD-Cl2B and pRHAB-Cl2B expressing RFPasv in two discrete expression cassettes, was also developed.
Genetic OR gates can be achieved by designing tandem promoter genetic circuits or by expressing target genes in two discrete expression cassettes.
All such fusions drove gfp expression in multiple locations suggesting that the different unc-62 transcripts are not responsible for discrete expression pattern components and all are expressed in many locations (WBID Expr9777/9778/9787/9793/9811/9818/9819/9825/9839/9845).
For the analysis of gene expression, the innovation lies in the discretization of the original expression values of genes into discrete expression state values and the definition of multigene expression state profiles.
However, only a single internal state in the HSPC attractor matched expression levels of all HSPC associated genes, whereas all others expressed different subsets of genes, suggesting possible heterogeneity between discrete expression states.
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Note that, if all the functionals (eta_{ij}) have discrete expressions, (1.2) gives a multi-point condition.
In engineering practice, the A-D test statistical variable A2 can be calculated from common discrete expressions (11).
The discrete expressions for the divergence of surface-type quantities (fluxes) are obtained with the help of discrete continuum analogy for the discrete balance equations.
Inconsistent discrete expressions in the boundary treatment of Navier Stokes solvers and in the definition of force objective functionals can lead to discrete-adjoint boundary treatments that are not a valid representation of the boundary conditions to the corresponding adjoint partial differential equations.
We conclude that our method, and indeed any statistically-principled method for cryptic initiation analysis using discrete expression-based probes, can more readily identify sites of cryptic initiation in long genes, where more probes flank every putative site.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com