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Comparison of the sequences of these two prospective retrotransposons revealed that they were closely related to one another and appeared to represent discrete copies of a novel family of retrotransposons.
Amplification was defined as greater than 10 discrete copies per nucleus or as a large gene copy cluster (confluent masses of more than 10 signals) in more than 50% of the nuclei evaluated (Tanner et al, 2000): we evaluated at least 400 cells in all cases.
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As a consequence of these arguments and using Lemma 2.4 we have the following proposition, a discrete copy of assertions we proved above.
We transformed continuous signal into discrete copy number to eliminate signal noise and slight batch bias.
R statistical software (version 2.5.0) was used to cluster intensity ratios and validate the cut-offs of the discrete copy number genotypes.
Most biological applications utilizing RNA, such as gene expression assays, generally rely on relative amounts of RNA as opposed to discrete copy numbers, hence the absolute amount of total RNA in a sample is of little importance [19].
Discrete copy numbers for each strain were determined by Sanger sequencing.
The observed continuous RD is considered to be generated by the hidden underlying discrete copy number states.
Our model explicitly represents the discrete copy number at a probe location as a latent random variable.
It should be noted that such discrete copy number classification is relative to the median CN in the references.
Discrete copy number profiles can also be queried as the most likely assignments of the latent copy number variables given observed data.
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