Although there is ample discrepancy in expression patterns of FN1, COL4A1, PAI-1, and TGFB1 in MC in response to HG among different reports, for the current study, we are confident that the methods utilized here, TaqMan qPCR and sandwich ELISA, are among the most sensitive and specific to quantify mRNA and proteins, respectively, thus, ensuring the accuracy of our findings.
In microbial organisms, genes in the same TU are considered co-transcribed, but in practice there might be some discrepancy in expression levels of the genes in the same TU due to the limits in microarray technology and intrinsic biological variation.
K-S -log10 P value, which provide a measure of the discrepancy in expression between SDN-enriched or SDN-less promoters and the rest of the promoters when ATP-dependent chromatin remodeler gene is mutated.
Further investigation will be needed to evaluate the impact of this discrepancy in expression profiles between presumed orthologous tobacco and poplar genes.
Thus, the simplest explanation of the developmental discrepancy in expression in the HPF is a sensitivity difference, with the MaxiPromoter driven lacZ being detected earlier than the ABA in situ hybridization for endogenous mouse transcripts.
The presence of the EBV genome in cells other than B lymphocytes may explain the discrepancy in relative expression of BHRF1-1 between B-cells and plasma.
In addition, there were discrepancies in expression pattern of some low abundant miRNAs during the infection detected by sequencing as compared to RT-PCR.
Discrepancies in expression profiles of some individual genes were largely due to platform differences and approaches to data analysis rather than to biological or interlaboratory variability.
Therefore, gross discrepancies in expression levels of these genes during parthenogenetic and meiotic reproduction in cyclical parthenogens are unlikely to be responsible for the mechanistic differences between these reproductive modes.
