There were a few discrepancies between methods that were most likely due to differences in the sensitivity of detection, and in the case of the macroarray, limitations in the sequence data available for certain virus species in the design of the oligonucleotides.
As in those studies, the exact basis for the occasional discrepancies between methods is not clear.
Discrepancies between methods for VISA phenotype definition have hampered standardization between studies (Satola et al. 2009; Satola, Farley, et al. 2011).
In this article we provided evidence that quantifies the existing discrepancies between methods whose performance is comparatively evaluated over different interactome datasets.
29 Whenever there are discrepancies between methods, IRSN recommends synergists be used together with biochemical methods for confirmation and determination of the possible resistance mechanisms in that particular population.
If the number of actions per minute is the source of discrepancies between methods, differences between the number of actions would be proportional to differences in indexes (Table 5).
Discrepancies between methods could either reflect differences in power or performance (i.e., approach could either over- or underestimate rates of selection) or reflect on real biological differences (i.e., rates of adaptation may have changed over time due to changes in ecology or other population parameters, such as population size).
Hence, it is not a straightforward task for a researcher to reason about the degree to which obtained results may be over- versus underestimated because he/she assumes that there was selective attrition and/or some discrepancies between method of analysis and RTM.
Since there was no discrepancy between the methods, we modified Ishiko's primers to convert the method from a semi-nested to a non-nested method for ease of use.
