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The single-copy integration of the transgenes into a specific locus allows direct comparison of gene-specific effects in the same genomic context and should reveal minimal discrepancies in expression.
Since microarray and SISH based comparisons are common for the validation of gene targets, a riboprobe design pipeline that minimizes discrepancies in expression validation arising from transcriptional complexity was created by consolidating the Affymetrix probeset locations into the design of SISH riboprobes.
Discrepancies in expression profiles of some individual genes were largely due to platform differences and approaches to data analysis rather than to biological or interlaboratory variability.
In addition, there were discrepancies in expression pattern of some low abundant miRNAs during the infection detected by sequencing as compared to RT-PCR.
Because lipasin is sensitive to nutritional status, it is not surprising that there are discrepancies in expression patterns, especially in humans, when the nutritional status is not strictly controlled.
Therefore, gross discrepancies in expression levels of these genes during parthenogenetic and meiotic reproduction in cyclical parthenogens are unlikely to be responsible for the mechanistic differences between these reproductive modes.
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We observed a clear discrepancy in expression of the RETN and CEBPE genes in myeloid samples newly obtained in vivo vs. the same cells conditioned in vitro.
Finally, the discrepancy in expression patterns between fish and terrestrial vertebrates may be due to the early, rapid evolution of the myelination process, which is crucial for vertebrate development.
Although there is ample discrepancy in expression patterns of FN1, COL4A1, PAI-1, and TGFB1 in MC in response to HG among different reports, for the current study, we are confident that the methods utilized here, TaqMan qPCR and sandwich ELISA, are among the most sensitive and specific to quantify mRNA and proteins, respectively, thus, ensuring the accuracy of our findings.
The discrepancy in expression quantitation for these outliers genes may reflect limitations in the measurement range for both technologies.
These results indicate that the discrepancy in expression changes might be a key factor during antibiotic biosynthesis [ 69].
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