Consequently, the final score indicates the number of discrepancies in the alignment.
Accessions of Phaius tancarvilleae deposited in GenBank had significant discrepancies in nucleotide alignment with some accessions suggesting translation using the aic [GenBank: KF852707, KF673844, KF673843] and others the cic [GenBank: AB04205, EF079306] (Additional file 4: Figure S3).
When a novel 16S type, mixed base pairs, or any discrepancies in the alignment were obtained, the 16S rRNA gene amplification and sequencing of the entire gene or parts containing the problematic region were repeated.
MIRA combines a redundancy based approach with a symbolic pattern analyzer developed for recognition of column discrepancies in sequence alignments to allow detection of SNPs.
In case of discrepancy in the alignment of the two linkage groups, we imposed the marker order from one parent as a fixed one for the other one and vice-versa.
Almost all the nucleotide discrepancies in the 24/25 alignments were at the SNP sites, which is an encouraging in silico validation of these SNPs.
Considering almost all the nucleotide discrepancies in the 24/25 alignments were at the SNP sites, it served well as in silico validation of these SNPs, in addition to and consistent with the rate (81%) validated by sequencing and SNaPshot assay.
Alignment uncertainty can cause discrepancies in phylogenetic inference [35], and possibly in other bioinformatic analyses.
Stars were in alignment, literally.
The stars have to be in alignment".
"All the stars were in alignment".
