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The European 'directive on the legal protection of biotechnological inventions' (EU/98/44), published in 1998, stipulated that the simple discovery of the sequence or partial sequence of a gene is not considered patentable.
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That this might be the case is supported by the discovery of the partial sequence Mbrevi5R3, which was manually constructed from unassembled genomic sequence reads.
J Biol Chem 1992 267 25010 8; Yoshida A, Suzuki M, Ikenaga H, Takeuchi M. Discovery of the shortest sequence motif for high level mucin-type O-glycosylation.
Discovery of the DNA sequence polymorphism is a prerequisite to generating genetic markers for various applications of modern genomics research such as map-based cloning, marker-assisted breeding, association mapping and understanding genome structure and function.
The discovery of these sequences will be helpful in studies on host-virus interactions.
Since the discovery of the consensus sequences, mutations in the surrounding regions have also been known to influence the output of gene expression, with vertical scaling of the expression response curve over as much as 3 4 orders of magnitude.
During DG allele discovery most of the sequences containing random sequencing errors were eliminated with the requirement that DG sequences used for genetic analysis are obtained at least three times from parental lines.
Recent developments in sequencing technology have simplified and accelerated the discovery of sequence variants, enabling the development of sequence-based markers including single nucleotide polymorphisms (SNPs) and insertion/deletion polymorphism (InDel) markers [ 14].
Then we step through elements of the workflow in greater detail, including a brief overview of the discovery of sequence variations from high-throughput sequence data.
The discovery of the amino acid sequence of BMP-1 prompted the research on its potential inhibitors [ 4, 29, 30].
Initially, the discovery of the chloroplast DNA sequence began with the physical mapping of the Zea mays chloroplast which was produced by digestion with multiple restriction enzymes [ 10].
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