We used this map to differentiate homoeologues based on their efficiency in amplifying F. vesca-derived markers and to discover genomic rearrangements among the diploid sub-genomes (homoeologues).
We calculated D on a genome-wide level using 5 kb, non-overlapping, sliding windows to discover genomic regions containing extended stretches of an excess of rare polymorphisms relative to the total number of segregating sites, indicating positive selection.
In general, they are used to (i) complete genome annotation [ 21], (ii) predict optimal culturing conditions [ 22- 24], (iii) discover genomic regulation [ 20, 25, 26], (iv) identify essential genes and drug targets [ 27- 33], (v) study strain evolution [ 34, 35], and (vi) design productive strains [ 36- 38].
We also discover genomic regions that exhibit unusually high levels of local chromatin interactions.
With the development of massive sequencing techniques and the availability of genome sequences, Genomic SELEX has the potential to be used as a very powerful selection procedure to discover genomic aptamers, i.e. sequences within RNAs that bind ligands with high affinity and are thus able to act as sensors or receptors within regulatory domains.
Using measurements from large ensembles of individual DNA molecules, we have discovered genomic structural alterations in the solid tumor oligodendroglioma.
We discovered genomic imprinting effects located on chromosome 19 with a strong effect on glycolytic potential and content.
GMTV helps to discover genomic variants of M. tuberculosis strains from different geographical areas and lists genetic markers associated with drug resistance and different clinical TB signs.
We performed linkage mapping to discover genomic loci that are associated with benzene-induced chromosomal damage in reticulocytes using DO mice in the combined 100-ppm exposure groups.
Summary: A tool to predict the effect that newly discovered genomic variants have on known transcripts is indispensible in prioritizing and categorizing such variants.
