The deep sequencing approach allowed us to discover and analyze the expression pattern of all the small RNAs that would not have been discovered using cloning based approach.
Additionally, in a series of immunohistochemistry experiments, we examined the expression patterns of critical molecules in this signaling pathway, including p-AKT, AKT, FoxO3a, p-FoxO3a, Bim and PTEN, and discovered that each of these proteins displayed a similar pattern as that seen in tumor cell lines.
This group has subsequently studied the expression patterns of all isoforms of CRMP (CRMP-1 to 5) and the proteolytic activity of calpain on CRMPs in ischemic brain injury, discovering that all CRMPs were highly expressed in apoptotic neurons [ 149].
It was also interesting to discover that TTP expression pattern was opposite to that of other TTR-RBPs, increasing with replicative senescence and decreasing in many tissues with advancing age.
Therefore, it is important to monitor the expression pattern of genes and to discover the genes that are coexpressed among multiple conditions.
Therefore, we aimed to discover the expression patterns that led to different phenotypes.
In addition to the high tissue preferences observed in previous studies, we also discovered two intriguing temporal expression patterns of the lineage-specific genes within zebrafish.
We discovered different temporal and spatial expression patterns of some immune-related genes in the Asian seabass after challenging with a pathogen Vibrio harveyi.
We also examined the gene expression patterns of the adult glomerular, medullary, and cortical endothelial cells, to discover the molecular basis of their compartment specific properties.
