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The scale of the movements undergone during a ribosome sliding event may be more similar to those documented in translational bypassing on the gene product 60 of bacteriophage T4 which is synthesized from a discontinuous reading frame (Samatova et al., 2014).
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In contrast, TAP-treated libraries showed discontinuous read distributions with peaks of coverage commonly occurring upstream of annotated genes.
The coding region consists of 3 discontinuous open reading frames (ORFs).
Lastly, a "non-unique, spliced" mapping approach is most commonly recognized and implemented in the TopHat v1.3.1 program [ 33], which includes the consideration of splice junctions for discontinuous mapping of reads.
Voles were analyzed from two discontinuous habitats, including three sites (ca. 20 km apart; Sample size: M1 = 41; M2 = 43; M3 = 41) at the Maodeng Livestock Farm (MD: GPS reading 44°11'N, 116°27'E), and three sites (ca. 25 km apart; Sample size: D1 = 44; D2 = 41; D3 = 42) in East Ujimqin (DWQ: GPS reading 45°44'N, 116°16'E).
It identifies probable spurs and chimers (reads that join discontinuous genomic loci) and trims each to one trusted clear range.
The method adopts a disorder TCP transmission strategy, which allows discontinuous data packets in TCP queues read by application layer directly without waiting for the retransmission of lost data packets.
We use the BLAST sample for both estimations, and reads are mapped using discontinuous BLASTN (NCBI BLAST 2.2.29+), which keeps matches with 80% minimum identity and only the best hit per read.
Importantly, we have also defined a concise format (BEDPE) to facilitate comparisons of discontinuous features (e.g. paired-end sequence reads) to each other (pairToPair), and to genomic features in traditional BED format (pairToBed).
Some of the originally defined deletion mutants exhibited the presence of 'internal' L er-0 reads within a 'Col-0 only' section, indicating the occurrence of discontinuous deletions.
We have therefore modified the referenced sentence to read: "To our knowledge, this is the first observation of clustered but discontinuous NCO gene conversion tracts in mammals, although patterns that resemble those shown in Figure 4A have been reported in meiosis (Tsaponina, 2014, Globus, 2013) and mitosis (Martini, 2011, St Charles, 2013) in S. cerevisiae".
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