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The electroencephalographic (EEG) background pattern of preterm infants changes with postmenstrual age (PMA) from discontinuous activity to continuous activity.
The model studied here may be a candidate mechanism for the generation of this temporal patterning in the discontinuous activity of the developing brain.
A discontinuous activity assay using benzoylarginine ethylester (BAEE) as a substrate was used to analyze citrulline production, using previously established methods.
EEG data are summarized in Table 2. Of note, of the 15 patients who died, 12 (75%) showed a nonreactive pattern and 11 (73%) prolonged discontinuous activity.
One had very rare spikes and waves missed with standard EEG, and the other became epileptiform only 12 hours after cEEG start; his EEG was started 12 h after cardiac arrest; ***one-sided 97.5% confidence interval During TH, as compared to cEEG data, the interpretation of sEEG recordings labeled one additional patient with discontinuous activity and a nonreactive background.
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A severely impaired EEG pattern or presence of a discontinuous background activity were also associated with a poor outcome (p = 0.015 and p = 0.002, respectively).
Among all parameters, a discontinuous background activity was the only variable that constantly predicted poor outcome (false-positive poor outcome prediction rate of 0%, 95% CI 0 15%).
Unless otherwise noted, all discontinuous glycosylase activity assays were performed by incubating AlkA and a DNA substrate at 37 °C in reaction buffer [50 mM NaMES (pH 6.1), 100 mM ionic strength (controlled with NaCl), 1 mM EDTA, 1 mM DTT, and 0.1 mg/mL BSA].
Presence of a discontinuous EEG background activity seems to be more accurate than clinical alterations to predict a bad neurologic outcome at 28 days.
By contrast, SARMs capturing discontinuous SARs or activity cliffs fall outside the QSAR applicability domain.
Nonreactive cEEG background (12 of 15 (75%) among nonsurvivors versus none of 19 (0) survivors; P < 0.001) and prolonged discontinuous "burst-suppression" activity (11 of 15 (73%) versus none of 19; P < 0.001) were significantly associated with mortality.
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