Sentence examples for discharged grid from inspiring English sources

When poly- l-lysine (PLL) was used to change the carbon surface of the EM grids, 1 mg/ml of low molecular weight PLL (Sigma P7890) was prepared in water and applied to a glow discharged grid for 10 min.

Samples were layered onto formvar coated/carbon coated/glow discharged copper grids and allowed to sit for 2 5 min. The grids were rinsed 3 times with water and stained with 2% uranyl acetate for 1 3 min. After removal of excess uranyl acetate solution, the grids were air-dried and examined at 60 kV in JEOL-1200EX.

Samples were prepared by applying the protein stock solution on hydrophilic glow discharged carbon grids that were negatively stained with 1% uranyl acetate (UA) or 2% (w/v) ammonium molybdate.

Glow discharging the grid to make the film more hydrophilic is a common routine for sample preparation and its efficiency is mainly depending on the property of the sample and how it is performed [ 59, 64].

In this stage, if the current selling price of electricity is higher than 80% of the maximum selling price of electricity during the day being simulated, and the SoC of the vehicle is above its minimum limit plus 10% and discharging is allowed at this time of the day according to the choice of the user in Table 3, then the EV will be discharged into the grid at the nominal rate (3 kW).

Formaldehyde-fixed polymer products at 0, 5, 8, and 12 min were diluted and transferred onto the 400 mesh, glow-discharged copper grids coated with Formvar/Carbon and then negatively stained with filtered 2% aqueous uranyl acetate essentially as described previously.

(A) About 3 μL of purified sample was applied to a newly glow-discharged grid coated with carbon film.

Five microliters of the assembly product was applied onto a glow-discharged grid that was held by self-closing, anti-capillary tweezers (EMS, Hatfield, PA, USA).

For the gold labelling, 3 μl FtsK-DNA complex were applied on glow discharged carbon-coated grids.

We applied 2.5 μl of the mixture to glow discharged holey carbon grids (QUANTIFOIL R 2/2) and vitrified in liquid ethane with an FEI Vitrobot.

3 µl of a 0.7 mg/ml Frh sample was applied to freshly glow discharged Quantifoil R1/4 grids (Quantifoil Micro Tools, Jena, Germany) that had been pretreated for 15 s in chloroform.