The surface of the head of the femur already consisted of a mixture of cement and amorphous necrotic bone, which no longer contained discernable cell nuclei.
In the liver, there was interindividual variation in the cellular distribution of reaction with staining present in all discernable cell types in most samples but confined to the hepatocytes and bile duct epithelial cells in others.
In the melanoma sections, all discernable cell types showed mainly nuclear staining: the intensity of staining varied between tissue samples and there was evidence of a range of intermediate staining intensities with some melanoma cells showing no detectable reaction.
The most prominent change for RSV-injected tumors, compared to medium-injected tumors, was complete necrosis with no histologically discernable tumor cells.
Since GFP+ axons of these explants could not be reliably imaged and quantified due to the low level of GFP expression, we approximated the extent of RET signaling in Gfra2 GFP DRG explants by quantifying the number of discernable GFP+ cell bodies.
Sections stained with H&E were used to visualize generalized chondrocyte cell death (determined by the amount of lacunae lacking discernable nuclei), localized cell death/focal cell loss (the presence of distinct regions devoid of discernable nuclei) and chondrocyte cluster formation (lacunae containing more than one nuclei).
There were no discernable differences in cell morphology between wild-type HEK 293 cells and cells transfected with vector alone.
Although miR-204 expression was significantly upregulated following transfection in all cell lines (Supplementary Figure 1), we observed no discernable effect on cell viability, as determined by MTS assays, relative to negative controls (Supplementary Figure 2A).
To exclude the possibility that a reduction of protein synthesis would be due to cytotoxic effects of digitoxin we chose a time point at which there were no discernable effects on cell viability.
There were no discernable changes in cell proliferation.
