A 50 μl blood sample was mixed with 1 ml lysis buffer (BD Biosciences, San Jose, CA, USA) in order to discard red cells and the mixture was then incubated at 37°C for 3 min.
In thinly veiled references to the US and India developing a joint containment strategy against an economically rising and increasingly militarist China, the US defence secretary said enhanced bilateral military ties and commerce could be fruitfully achieved by discarding "bureaucratic red tape".
The narrative is overlong, with discarded subplots and red herrings, but Simmons, a master of otherworldly suspense, cleverly explores envy's corrosive effects.
The encounter came after the protesters — who have largely discarded their trademark red shirts — made a rare foray from their camps in the center of Bangkok, where they have forced the closing of shopping malls and major hotels.
We mixed the blood by gentle inversion of the tube and centrifuged it at 1,200 × g for 10 min. The plasma supernatant was discarded and the red blood cells (RBCs) were washed 4 times by suspending them in saline (0.9%) before centrifugation at 1,200 × g for 10 min. The final suspension consisted of 5% by volume RBC in saline.
In forging the deal with Nick Clegg, Cameron had to discard the reddest of those pieces.
She finds a discarded raw chicken, still in its plastic wrapping, and hands it down to a little red-haired boy.
Over the years among the detritus, we found a plastic Christmas wise man, ring settings, a finger (rumored), a floppy dildo in a red van, discarded elastics, keychains, snapshots, bills and letters.
