There was a 91% probability that nurse-led follow-up was cost-effective compared to standard follow-up, discarding quality-of-life effects (willingness to pay €0, Figure 3).
We aggressively trimmed, corrected, and discarded lower quality reads and assembled those satisfying our quality control criteria using SOAPdenovo (Li et al. 2010) and a custom parameter optimization strategy (see Materials and Methods).
In all platforms, save for Nimblegen, some hybridizations were discarded under quality control procedures.
We also discarded poor quality data and took steps to achieve theoretical and procedural rigor.
In the present study, we discarded low quality samples from scoring, ran negative controls, and performed a repeatability test on nine previously typed individuals.
In a larger scale study, samples such as this one could be discarded during quality control, but it is important to note that even in this sample, 3432 of the 4102 loci assessed gave scoreable and repeatable genotypes.
The heteroplasmy detection threshold was set to 0.2%; the sensitivity and specificity of detected heteroplasmies were determined from 12 samples (one sample was discarded after quality filters) whose levels of heteroplasmy were measured using 3 different PCR products.
The attack piece also claims that the scientists discarded high-quality temperature measurements in favor of low quality data.
Performing trimming on NGS data not only cuts off the low quality bases from both ends, but also discards poor quality reads, and thus improving reads' quality markedly.
We discarded low-quality reads, which contained adapters and unknown or low-quality bases, according to our bioinformatics analysis.
