After the image segmentation by DBSCAN, the regions that were substantially smaller than the pellets too dark or contacting the image border were discarded from further analysis.
In addition, SNP fixed for the same allele in all populations were discarded from further analysis.
Thirteen animals genotyped on less than 90% of the SNPs were discarded from further analysis.
The results of such trials (<10%) were discarded from further analysis.
After taking the relatedness into account, 13 samples were discarded from further analysis.
Probe sets showing significant batch and interaction effects (p-value< = 0.001) were discarded from further analysis.
Alignments showing more than 10% site gaps were also discarded from further analysis (Table S1).
Sequences containing more than 3 mismatches with any reported sequence were discarded from further analysis.
As a further quality control step, arrays with an overall call rate less than 98% were discarded from further analysis.
Mutations with descriptions that did not match any of the given strings were discarded from further analysis.
Clones that did not confirm the growth and blue color production when co-transformed with their bait septins were discarded from further analysis (false positives).
