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After pupil dilation (topical solution of tropicamide 1%), a 45° retinal photograph of one eye, centered on the region of the optic disc, was acquired.
Spinning disc confocal data was acquired on an UltraView RS3 (Perkin-Elmer Systems) fitted on a Zeiss Axiovert 200M microscope with illumination from a 488 nm Argon laser (Melles Griot).
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Thermal fatigue crack propagation was acquired for evaluating the safety degree of the brake disc due to emergency braking mode.
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Eight serial T2-weighted sagittal images covering the entire disc area were acquired using a spin-echo sequence with the following parameters: fat saturation on; repetition time/effective echo time, 3000/30 ms; field of view, 30 × 60 mm; matrix, 128 × 128; slice thickness, 0.5 mm; slice spacing, 0 mm; number of slices, eight; number of scans, 1 (total scan time 6.24 minutes).
Spinning disc confocal data were acquired with an Ultra View spinning disc confocal (Perkin Elmer, Waltham MA) on a Zeiss Axiovert 200M.
Spinning disc confocal images were acquired with a Perkin Elmer confocal box and an OrcaER CCD camera on a Nikon inverted stand with a 100× oil immersion 1.4-numerical aperture objective.
Three consecutive scans of the optic disc and macular region were acquired and analysed for each eye.
