Sentence examples for disc unit from inspiring English sources
The emitted light was detected after passage through the Nipkow disc unit with a CCD camera (Orca ERG, Hamamatsu, Japan).
We also performed an analysis of variance on the number of cells undergoing mitosis per wing disc unit area for each population (Fig. 4).
Multiplexed Optically Sectioned FLIM Microscope: This microscope utilised essentially the same experimental setup as described in ref. [ 27] except that the manually configurable Nipkow disc unit (Yokogawa CSU-10) was replaced by the newly available Nipkow disc unit (Yokogowa, model CSU-X A3) that provides improved light efficiency and electronic switching of the dichroic beam splitters.
We have now demonstrated an optically sectioned multiplexed FLIM microscope based on the approach of our earlier work[ 41] but using a Nipkow disc unit incorporating an electronically controlled dichroic changer and electronically controlled excitation and emission filter wheels.
Images were taken with a 60× objective on a Nikon inverted Ti-E microscope with a Yokagawa spinning disc unit and an EM-CCD camera (Hamamatsu ImagEM); Citrine was excited with a 488-nm laser; exposure times were 200 ms with a time interval of 2 or 3 min. Typically, 16 positions were imaged for 8 hr per experiment.
After fixation, disc units were serially dehydrated in alcohol.
Whole disc units, including the endplates, were fixed in 10% formaldehyde in PBS for routine histological and immunohistochemical evaluation.
Images were acquired on an Olympus 1X81 fluorescence microscope equipped with a CSU-X1 spinning-disc unit (YOKOGAWA) or a Nikon-A1 laser scanning confocal microscope (Nikon).
There are probably more working eight-track tape players than there are laser-disc units.
Epifluorescence images were captured with the similar system as described above but without the spinning disc confocal unit.
Confocal three-dimensional images were taken by using Zeiss Axiovert 200 M with spinning disc confocal unit Yokogawa CSU22 and a Zeiss Plan-Neofluar 5× objective.
